Cite as:
Well, R.; Burkart, S.; Giesemann, A.; Grosz, B.; K&ouml;ster, J.R. &amp; Lewicka-Szczebak, D. (2018): <b>Improvement of the 15N gas flux method for in situ measurement of soil denitrification and its product stoichiometry </b>. <i>Rapid Communications in Mass Spectrometry</i> <b>33</b>, 437-448<br>DOI: <a href="" target="_blank"></a>.

Resource Description

Title: Improvement of the 15N gas flux method for in situ measurement of soil denitrification and its product stoichiometry
FOR816dw ID: 10
Publication Date: 2018-11-25
License and Usage Rights: DASIM data user agreement. (
Resource Owner(s):
Individual: Reinhard Well
Individual: Stefan Burkart
Individual: Anette Giesemann
Individual: Balázs Grosz
Individual: Jan Reent Köster
Individual: Dominika Lewicka-Szczebak
RATIONALE<br/> Field measurement of denitrification in agricultural ecosystems using the 15N gas flux method has been limited by poor sensitivity because current isotope ratio mass spectrometry is not precise enough to detect low 15N2 fluxes in the presence of a high atmospheric N2 background. For laboratory studies, detection limits are improved by incubating soils in closed systems and under N2?depleted atmospheres.<br/> METHODS<br/> We developed a new procedure to conduct the 15N gas flux method suitable for field application using an artificially N2?depleted atmosphere to improve the detection limit at the given precision of mass spectrometry. Laboratory experiments with and without 15N?labelling and using different flushing strategies were conducted to develop a suitable field method. Subsequently, this method was tested in the field and results were compared with those from the conventional 15N gas flux method.<br/> RESULTS<br/> Results of the two methods were in close agreement showing that the denitrification rates determined were not biased by the flushing procedure. Best sensitivity for N2+N2O fluxes was 10 ppb, which was 80?fold better than that of the reference method. Further improvement can be achieved by lowering the N2 background concentration below the values established in the present study.<br/> CONCLUSIONS<br/> In view of this progress in sensitivity, the new method will be suitable to measure denitrification dynamics in the field beyond peak events.
| 15N tracing | denitrification | field measurement | method development |
Literature type specific fields:
Journal: Rapid Communications in Mass Spectrometry
Volume: 33
Page Range: 437-448
Metadata Provider:
Individual: Kristina Kleineidam
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